UNIT V Affinity chromatography
Affinity chromatography
Introduction, theory, instrumentation and applications
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UNIT – I UV Visible spectroscopy
UNIT – I UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component analysis Follow this Link for presentation Follow this Link for the Calculation of 𝝀max Woodward–Fieser rules Follow this Link for study materials from open sources This AI-generated visual takes you through t he working principle of UV–Visible spectrophotome try—from the light source and monochromator to sample interaction, detection, and generation of the absorption spectrum.
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