UNIT – I UV Visible spectroscopy

 UNIT – I  

UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. 

Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications - Spectrophotometric titrations, Single component and multi component analysis

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Follow this Link for the Calculation of 𝝀max 

Woodward–Fieser rules

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This AI-generated visual takes you through the working principle of UV–Visible spectrophotometry—from the light source and monochromator to sample interaction, detection, and generation of the absorption spectrum.