UNIT – I UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations. Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component analysis Follow this Link for presentation Follow this Link for the Calculation of 𝝀max Woodward–Fieser rules Follow this Link for study materials from open sources
Unit III Introduction to chromatography Gas chromatography Gas chromatography - Introduction, theory, instrumentation, derivatization, temperature programming, advantages, disadvantages and applications Follow this Link for presentation Follow this Link for presentation on Derivatization in GC Follow this Link for study materials from open sources Follow this Link for YouTube
UNIT – I Fluorimetry Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications Follow this Link for presentation Follow this Link for study materials from open sources
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