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UNIT – I Fluorimetry

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  UNIT – I   Fluorimetry  Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications Follow this Link for presentation Follow this Link for study materials from open sources

UNIT – I UV Visible spectroscopy

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 UNIT – I   UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.  Instrumentation - Sources of radiation, wavelength selectors, sample cells, detectors- Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode. Applications - Spectrophotometric titrations, Single component and multi component analysis Follow this Link for presentation Follow this Link for the  Calculation of  𝝀max  Woodward–Fieser rules Follow this Link for study materials from open sources

Syllabus

 BP701T. INSTRUMENTAL METHODS OF ANALYSIS (Theory) Scope:  This subject deals with the application of instrumental methods in qualitative and quantitative analysis of drugs. This subject is designed to impart a fundamental knowledge on the principles and instrumentation of spectroscopic and chromatographic technique. This also emphasizes on theoretical and practical knowledge on modern analytical instruments that are used for drug testing. Objectives:  Upon completion of the course the student shall be able to 1. Understand the interaction of matter with electromagnetic radiations and its applications in drug analysis 2. Understand the chromatographic separation and analysis of drugs. 3. Perform quantitative & qualitative analysis of drugs using various analytical instruments. Unit I                                                                                              (10 Hours) UV Visible spectroscopy Electronic transitions, chromophores, auxochromes, spectral shifts, solve